Specialized Colorimeter for Phenol red pH Measurement

Application of a colorimeter for turbidity measurement

Antagonism of ATP responses at P2X receptor subtypes by the pH indicator dye, Phenol red.

1 Many types of culture media contain a pH-sensitive dye. One commonly occurring dye, Phenol red sodium (Na(+)) salt, was tested for blocking activity at rat P2X(1-4) receptors (P2X(1-4)Rs) expressed in Xenopus oocytes. 2 Phenol red Na(+)-salt antagonised adenosine 5'-triphosphate (ATP) responses at P2X(1)R (IC(50), 3 microM) and, at higher concentrations, also blocked P2X(2)R and P2X(3)R. Phen...

Absorbance signals from resting frog skeletal muscle fibers injected with the pH indicator dye, phenol red

Singly dissected twitch fibers from frog muscle were studied on an optical bench apparatus after micro-injection with the pH indicator dye, phenol red. Dye-related absorbances in myoplasm, denoted by A0(lambda) and A90(lambda), were estimated as a function of wavelength lambda (450 nm less than or equal to lambda less than or equal to 640 nm) with light polarized parallel (0 degrees) and perpen...

The Phenol Red Clearance in Normal Man.

Although phenol red, introduced by Rowntree and Geraghty (6) in 1912, has been widely used in man as an empirical renal function test, the excretion of this substance relative to its concentration in the plasma has been examined in only one instance. MacKay (1930) injected one gram intravenously and calculated the Addis excretory ratio in three successive periods when the plasma level was 5.80,...

Biodegradation of Phenol by Newly Isolated Phenol-degrading Bacterium Ralstonia sp. Strain PH-S1

A newly phenol-degrading bacterium, identified as Ralstonia sp. strain PH-S1, was isolated from oil-contaminated soil in Khark Island. It was isolated by a multistep enrichment and screening technique on mineral medium (MM) containing 100 mg.l-1 of phenol as the sole source of carbon. The bacterium was able to degrade up to 1100 mg.l-1 of phenol but the cell growth decreased with higher concent...